Immunostimulatory and Anti-inflammatory Effect of Ganoderma Lucidum on Breast Cancer Patients

Ramadhan Othman

Abstract


Background

Breast cancer (BC) is the most common cancer among women. A key factor in tumour development is the evasion of immune detection therefore; search for alternative medicines with the ability to improve function of immune system and targets tumours cells has received growing interest. Ganoderma lucidum (GL) has been demonstrated to possess anti-tumour and immunomodulatory activities. Little is known about immunomodulatory effect of GL on BC patients when used with chemotherapeutic agents. We aim to investigate the level of immune biomarkers; interferon-γ (IFN-γ), tumour necrotic factor-α (TNF-α), inteleukin-8 (IL-8) and adiponectin in these patients.

Material and Methods

Forty female patients with BC were included and divided equally into two groups: Group 1 received chemotherapy alone and Group 2 received chemotherapy plus GL capsules (1000 mg twice daily). Blood samples were obtained from all patients before and after 4 cycles of chemotherapy. These samples were analysed to identify the levels of IFN-γ, TNF-α, IL-8 and adiponectin.

 

Results

After the treatment period, there was significant increase in the level of IFN-γ (P=0.0024) and a significant decrease in the mean serum levels of TNF-α (P=0.0004) and IL-8 (P=0.019) in patients treated with concurrent GL and chemotherapy. A non-significant difference was found between pre and post treatment in the mean serum levels of TNF-α, IFN-γ and IL-8 in patients received chemotherapy only. The mean serum levels of adiponectin in both groups did not show any significant difference when compared to pre-treatment levels.

Conclusion

The present study showed that the use of GL in-combination with chemotherapy could have immune stimulatory, anti-inflammatory and anti-metastatic activity that can enhance therapeutic responsiveness of BC patients to chemotherapy and improve their quality of life during treatment. More research is needed to confirm these results. In addition, clinical evaluation of benefit and outcome are on going

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References

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA: a cancer journal for clinicians. 2015;65(2):87-108.

Siegel RL, Miller KD, Jemal A. Cancer statistics, 2016. CA: a cancer journal for clinicians. 2016;66(1):7-30.

Othman RT, Abdulljabar R, Saeed A, Kittani SS, Sulaiman HM, Mohammed SA, et al. Cancer incidence rates in the Kurdistan region/Iraq from 2007-2009. Asian Pacific journal of cancer prevention : APJCP. 2011;12(5):1261-4.

Pusztai L, Karn T, Safonov A, Abu-Khalaf MM, Bianchini G. New Strategies in Breast Cancer: Immunotherapy. Clinical cancer research : an official journal of the American Association for Cancer Research. 2016;22(9):2105-10.

Wang M, Zhang C, Song Y, Wang Z, Wang Y, Luo F, et al. Mechanism of immune evasion in breast cancer. OncoTargets and therapy. 2017;10:1561-73.

Bracci L, Schiavoni G, Sistigu A, Belardelli F. Immune-based mechanisms of cytotoxic chemotherapy: implications for the design of novel and rationale-based combined treatments against cancer. Cell death and differentiation. 2014;21(1):15-25.

Vichaya EG, Chiu GS, Krukowski K, Lacourt TE, Kavelaars A, Dantzer R, et al. Mechanisms of chemotherapy-induced behavioral toxicities. Frontiers in neuroscience. 2015;9:131.

Vyas D, Laput G, Vyas AK. Chemotherapy-enhanced inflammation may lead to the failure of therapy and metastasis. OncoTargets and therapy. 2014;7:1015-23.

Jiang J, Sliva D. Novel medicinal mushroom blend suppresses growth and invasiveness of human breast cancer cells. International journal of oncology. 2010;37(6):1529-36.

Bishop KS, Kao CH, Xu Y, Glucina MP, Paterson RR, Ferguson LR. From 2000years of Ganoderma lucidum to recent developments in nutraceuticals. Phytochemistry. 2015;114:56-65.

Xu Z, Chen X, Zhong Z, Chen L, Wang Y. Ganoderma lucidum polysaccharides: immunomodulation and potential anti-tumor activities. The American journal of Chinese medicine. 2011;39(1):15-27.

Martinez-Montemayor MM, Acevedo RR, Otero-Franqui E, Cubano LA, Dharmawardhane SF. Ganoderma lucidum (Reishi) inhibits cancer cell growth and expression of key molecules in inflammatory breast cancer. Nutrition and cancer. 2011;63(7):1085-94.

Tung NT, Cuong TD, Hung TM, Lee JH, Woo MH, Choi JS, et al. Inhibitory effect on NO production of triterpenes from the fruiting bodies of Ganoderma lucidum. Bioorganic & medicinal chemistry letters. 2013;23(5):1428-32.

Batbayar S, Kim MJ, Kim HW. Medicinal mushroom Lingzhi or Reishi, Ganoderma lucidum (W.Curt.:Fr.) P. Karst., beta-glucan induces Toll-like receptors and fails to induce inflammatory cytokines in NF-kappaB inhibitor-treated macrophages. International journal of medicinal mushrooms. 2011;13(3):213-25.

Guggenheim AG, Wright KM, Zwickey HL. Immune Modulation From Five Major Mushrooms: Application to Integrative Oncology. Integrative medicine (Encinitas, Calif). 2014;13(1):32-44.

Wu GS, Guo JJ, Bao JL, Li XW, Chen XP, Lu JJ, et al. Anti-cancer properties of triterpenoids isolated from Ganoderma lucidum - a review. Expert opinion on investigational drugs. 2013;22(8):981-92.

Yue QX, Xie FB, Guan SH, Ma C, Yang M, Jiang BH, et al. Interaction of Ganoderma triterpenes with doxorubicin and proteomic characterization of the possible molecular targets of Ganoderma triterpenes. Cancer science. 2008;99(7):1461-70.

Thyagarajan A, Jedinak A, Nguyen H, Terry C, Baldridge LA, Jiang J, et al. Triterpenes from Ganoderma Lucidum induce autophagy in colon cancer through the inhibition of p38 mitogen-activated kinase (p38 MAPK). Nutrition and cancer. 2010;62(5):630-40.

Chen X, Hu ZP, Yang XX, Huang M, Gao Y, Tang W, et al. Monitoring of immune responses to a herbal immuno-modulator in patients with advanced colorectal cancer. International immunopharmacology. 2006;6(3):499-508.

Gao Y, Tang W, Dai X, Gao H, Chen G, Ye J, et al. Effects of water-soluble Ganoderma lucidum polysaccharides on the immune functions of patients with advanced lung cancer. Journal of medicinal food. 2005;8(2):159-68.

Critchley-Thorne RJ, Simons DL, Yan N, Miyahira AK, Dirbas FM, Johnson DL, et al. Impaired interferon signaling is a common immune defect in human cancer. Proceedings of the National Academy of Sciences of the United States of America. 2009;106(22):9010-5.

Gao Y, Zhou S, Jiang W, Huang M, Dai X. Effects of ganopoly (a Ganoderma lucidum polysaccharide extract) on the immune functions in advanced-stage cancer patients. Immunological investigations. 2003;32(3):201-15.

Zhou XL, Fan W, Yang G, Yu MX. The clinical significance of PR, ER, NF- kappa B, and TNF- alpha in breast cancer. Disease markers. 2014;2014:494581.

Korobeinikova E, Myrzaliyeva D, Ugenskiene R, Raulinaityte D, Gedminaite J, Smigelskas K, et al. The prognostic value of IL10 and TNF alpha functional polymorphisms in premenopausal early-stage breast cancer patients. BMC genetics. 2015;16:70.

Jiang J, Grieb B, Thyagarajan A, Sliva D. Ganoderic acids suppress growth and invasive behavior of breast cancer cells by modulating AP-1 and NF-kappaB signaling. International journal of molecular medicine. 2008;21(5):577-84.

Zhao H, Zhang Q, Zhao L, Huang X, Wang J, Kang X. Spore Powder of Ganoderma lucidum Improves Cancer-Related Fatigue in Breast Cancer Patients Undergoing Endocrine Therapy: A Pilot Clinical Trial. Evidence-based complementary and alternative medicine : eCAM. 2012;2012:809614.

Argiles JM, Lopez-Soriano FJ. The role of cytokines in cancer cachexia. Medicinal research reviews. 1999;19(3):223-48.

Mendes MC, Pimentel GD, Costa FO, Carvalheira JB. Molecular and neuroendocrine mechanisms of cancer cachexia. The Journal of endocrinology. 2015;226(3):R29-43.

Haslett PA. Anticytokine approaches to the treatment of anorexia and cachexia. Seminars in oncology. 1998;25(2 Suppl 6):53-7.

Waugh DJ, Wilson C. The interleukin-8 pathway in cancer. Clinical cancer research : an official journal of the American Association for Cancer Research. 2008;14(21):6735-41.

Bieche I, Chavey C, Andrieu C, Busson M, Vacher S, Le Corre L, et al. CXC chemokines located in the 4q21 region are up-regulated in breast cancer. Endocrine-related cancer. 2007;14(4):1039-52.

Thyagarajan A, Jiang J, Hopf A, Adamec J, Sliva D. Inhibition of oxidative stress-induced invasiveness of cancer cells by Ganoderma lucidum is mediated through the suppression of interleukin-8 secretion. International journal of molecular medicine. 2006;18(4):657-64.

Lai KN, Chan LY, Tang SC, Leung JC. Ganoderma extract prevents albumin-induced oxidative damage and chemokines synthesis in cultured human proximal tubular epithelial cells. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association. 2006;21(5):1188-97.

Shi Z, Yang WM, Chen LP, Yang DH, Zhou Q, Zhu J, et al. Enhanced chemosensitization in multidrug-resistant human breast cancer cells by inhibition of IL-6 and IL-8 production. Breast cancer research and treatment. 2012;135(3):737-47.

Derin D, Soydinc HO, Guney N, Tas F, Camlica H, Duranyildiz D, et al. Serum IL-8 and IL-12 levels in breast cancer. Medical oncology (Northwood, London, England). 2007;24(2):163-8.

Dudhgaonkar S, Thyagarajan A, Sliva D. Suppression of the inflammatory response by triterpenes isolated from the mushroom Ganoderma lucidum. International immunopharmacology. 2009;9(11):1272-80.

Zhu XL, Chen AF, Lin ZB. Ganoderma lucidum polysaccharides enhance the function of immunological effector cells in immunosuppressed mice. Journal of ethnopharmacology. 2007;111(2):219-26.

Ohashi K, Parker JL, Ouchi N, Higuchi A, Vita JA, Gokce N, et al. Adiponectin promotes macrophage polarization toward an anti-inflammatory phenotype. The Journal of biological chemistry. 2010;285(9):6153-60.

Tworoger SS, Eliassen AH, Kelesidis T, Colditz GA, Willett WC, Mantzoros CS, et al. Plasma adiponectin concentrations and risk of incident breast cancer. The Journal of clinical endocrinology and metabolism. 2007;92(4):1510-6.

Shahar S, Salleh RM, Ghazali AR, Koon PB, Mohamud WN. Roles of adiposity, lifetime physical activity and serum adiponectin in occurrence of breast cancer among Malaysian women in Klang Valley. Asian Pacific journal of cancer prevention : APJCP. 2010;11(1):61-6.

Matthews SB, Thompson HJ. The Obesity-Breast Cancer Conundrum: An Analysis of the Issues. International journal of molecular sciences. 2016;17(6).




DOI (PDF (FULL TEXT)): http://dx.doi.org/10.22034/APJCB.2018.3.2.51

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